Intrinsic terminators in Mycoplasma hyopneumoniae transcription

BMC Genomics

Table 4 Predicted terminators analysed by qPCR

Terminator	Validation ¹	Localisation	Class	Gene ²	Distance (bp) ³	ΔG ⁴
w_016	TD	mC_04	t_c3	rbgA	88	−6.06
w_053	TD	mC_15	t_c3	pyrG	82	−5.01
w_082	No	mC_24	t_c3	gyrA	75	−6.41
wf_204	TD	mC_30	t_c4	pulA	131	−2.48
w_109	TE	TU_009	t_c2	fusA	39	−17.8
t_022	TD	TU_011	t_c2	deoA	119	−10
t_055	TE	TU_020	t_c2	rpmB	49	−7.6
w_140	TD	TU_031	t_c3	trmE	35	−7.81
w_055	TD	TU_047	t_c3	ugpQ	18	−8.9
a_207	TD	TU_058	t_c2	hit	27	−5.8
t_173	TD	TU_062	t_c2	MHP7448_0372	43	−9.4
wf_140	TD	TU_068	t_c3	ruvB	42	−3.91
w_181	TD	TU_077	t_c3	rplA	107	−6.19
a_310	TD	TU_083	t_c2	MHP7448_0494	24	−5.12
w_093	TD	TU_105	t_c2	MHP7448_0628	109	−8.35
w_102	TE	TU_113	t_c2	MHP7448_0665	43	−11

¹Presence of functional activity. TE – transcription termination; TD – transcription decreasing; No – without difference in transcript upstream and downstream of the terminator sequence.
²Gene at the end of transcription unit.
³Distance between stop codon of the gene and the first nucleotide of terminator sequence.
⁴Value of free energy of Gibbs identified by the software that predicted the terminator.

ISSN: 1471-2164