Skip to main content

Table 2 Depth information of each allele and each locus

From: Cost-efficient multiplex PCR for routine genotyping of up to nine classical HLA loci in a single analytical run of multiple samples by next generation sequencing

Locus

Average depth (average ± SD)

Average depth ratio (average ± SD)*

Average depth per locus (average ± SD)

 

Observed

Normalized*

 

Observed

Normalized*

HLA-A

78.5 ± 42.0

78.2 ± 37.9

1.0 ± 0.1

156.9 ± 48.5

156.3 ± 36.2

HLA-B

116.5 ± 51.1

117.8 ± 46.8

0.9 ± 0.2

233.0 ± 75.2

235.6 ± 68.2

HLA-C

130.0 ± 59.3

127.4 ± 55.8

0.9 ± 0.1

257.1 ± 90.9

255.4 ± 71.3

HLA-DRB1

209.1 ± 115.9

208.4 ± 108.2

0.9 ± 0.3

418.3 ± 143.0

416.7 ± 114.3

HLA-DQB1

194.7 ± 104.9

194.4 ± 95.3

0.9 ± 0.1

389.3 ± 115.9

388.9 ± 100.4

HLA-DPB1

59.2 ± 34.6

59.2 ± 31.3

0.9 ± 0.1

116.3 ± 42.6

118.4 ± 37.8

  1. *calculated using the average draft read number (114,882 reads) shown in Additional file 2: Table S1.