Figure 5

Subcellular localizations of ParBc-sGFP and ParBm-sGFP in T. thermophilus and E. coli cells. Representative cells are shown with a gallery view of phase-contrast (Phase), DNA, ParBc-sGFP or ParBm-sGFP signal, and merged images (Overlay). Scale bars, 2 μm. (A and F) Subcellular localization of ParBc-sGFP and ParBm-sGFP in the T. thermophilus TL-1 strain grown in complex medium. (B, C, D and H) Expression of T. thermophilus ParBc-sGFP or ParBm-sGFP in E. coli XL-1. In the absence of the parSc site, ParBc-sGFP was found as patches (B); when parSc sites were provided from a plasmid, ParBc-sGFP localized as discrete foci (C), and foci were not observed in the presence of the empty vector (D); ParBm-sGFP formed discrete foci in E. coli (H). (G) Representative image of mislocalized foci formed by ParBm-sGFP expressed in the ΔparAmN-1 strain. (E and I) Relative positions of the two most pole-proximal foci of ParBc-sGFP (E) and ParBm-sGFP (I) expressed in T. thermophilus TL-1. Black diamonds represent the nearest-to-pole foci positions in individual cells, white diamonds represent the foci positions that are farthest from these poles. The mean position of the nearest-to-pole foci is shown with a dotted line. (J) The relative foci positions of 120 ΔparAmN-1/ParBm-sGFP cells containing one focus are shown.