Figure 4

Light microscopy of nematocysts used in proteomic analysis and SDS-PAGE gels of venom proteins. Two nematocyst preparations were analyzed; a preparation containing predominantly mastigophores (right) and a preparation enriched in isorhizas and trirhopaloids (left). Different morphological types are indicated (magnification 200x). Extracts of the nematocyst preparations were fractionated using SDS-PAGE (bottom) and proteins identified using tandem mass spectrometry. Forty-one gel slices were excised from each lane, as indicated by the aligned metal grid (right). Selected gel slices corresponding to major protein bands are indicated on the gel. Where a protein band was divided between two gel slices, an asterisk denotes the gel slice containing the majority of that protein.