Fig. 5

HIV gp120 up-regulates PIAS3 and STAT1 protein expression in MDDCs. MDDCs were stimulated with gp120 (5 μg/ml) for 18 h. Cell lysates were resolved by 8-12 % SDS-PAGE, transferred to a nitrocellulose membrane, and subjected to immunoblot analysis with antibodies specific for: p38, ERK1/2, JNK, p65, p50, IkBα, IKKβ, JAK2, STAT-1, −2, −3, SOCS-1, −3, PIAS3, Bcl2, BCLxL, Akt, and PKR. Data from one representative experiment are shown. a Graphs show the level of protein as determined by densitometry (ImageJ software) and calculated relatively to untreated control (C), where each sample was normalized to total actin. The average fold from two-four independent experiments was represented, along with the SD. b Immunoblot analysis with antibodies specific for PIAS3 and STAT1. Actin expression is shown as gel loading control. Values below the lanes show band intensities of the respective bands, normalized to actin expression. Data from one representative experiment out of four analyzed are shown