Fig. 1

ChIP-Seq reveals NEUROD2 target genes during cortical development. a Chromatin DNA was immunoprecipitated with three different antibodies against NEUROD2 (ab1, ab2 or ab3) or with a GFP antibody which was used as a negative control. PCR was performed using immunoprecipitated DNA as template. Specific primers amplified the promoter region of a previously identified NEUROD2 target gene Nhlh2 [16]. An enrichment for the positive control Nhlh2 gene was demonstrated in NEUROD2 ChIP-PCR samples as compared to GFP ChIP-PCR. b The developing cortex (dorsal telencephalon) was dissected from E14.5 mouse brain and used for NEUROD2 ChIP-Seq. 32,367 peak regions and 1,045 target genes were identified