Fig. 1
From: cis-regulatory analysis of the Drosophila pdm locus reveals a diversity of neural enhancers
The pdm locus and the evolutionary relationship of its encoded pdm proteins. a An alignment of the long and short isoforms of nub and pdm-2 genes to a UCSC genome browser histogram along the left arm of the 2nd chromosome (chr2L). Peaks indicate degrees of evolutionary conservation among 12 Drosophila species. b Clustal alignment of Dipteran POU protein sequences including the short isoforms of Nubbin and Pdm-2 from D. melanogaster, Musca domesticus (housefly), Anopheles gambiae and Culex quinquefasciatus (mosquito) and Ceratitis capitata (Mediterranean fly). The D. melanogaster POU homeodomain transcription factors Ventral veins lacking (Vvl) and Abnormal chemosensory jump 6 (Acj6) amino acid sequences were included as outgroup comparisons. Alignment was carried out using Clustal W2 server of Kyoto University Bioinformatics Center (http://www.genome.jp/tools/clustalw/). The tree was constructed using the UPGMA (Unweighted Pair Group Method with Arithmetic Mean) algorithm