Fig. 1

Ae. aegypti life cycle and adult behaviors. a Schematic of the mosquito life cycle and the female gonotrophic cycle. b Female host-seeking behavior at three stages of the gonotrophic cycle as assayed in a uniport olfactometer [12] (ANOVA followed by Tukey’s HSD, all differences p < 0.05, n = 8–10 per condition). c Eggs laid in 8 h after females were offered an oviposition substrate at 48 or 96 h after a blood-meal (Welch two-sample t-test, p < 0.001; n = 50 females). d Response to CO₂ assayed in a three dimensional flight tracking arena [8]. Mean indicated with the black line and S.E.M. by the gray shading; n = 4–5 per condition. e Summary of flight tracking data, with distance flown quantified in 6-min periods prior to and following a 40 s pulse of CO₂. Post-CO₂ activity of blood-fed females is reduced as compared to all other conditions (ANOVA followed by Tukey’s HSD; p < 0.05), and pre-CO₂ activity of gravid females is increased as compared to all other conditions (ANOVA followed by Tukey’s HSD; p < 0.05)