Fig. 9

Gene expression changes across the female gonotrophic cycle. MA plots of genes differentially expressed in non-blood-fed versus blood-fed females (a, c, e) or non-blood-fed versus gravid females (b, d, f) in brain (a and b), antenna (c and d) and hindleg (e and f). Genes were identified as significantly regulated within a single tissue using DESeq2 (α < 0.01). g Z-scores of significantly regulated genes (α < 0.01) of either non-blood-fed versus blood-fed or non-blood-fed versus gravid) were clustered into 6 qualitatively distinct clusters, four of which are shown as heat maps (h–k) Abundances of example genes from brain (h) and antenna (i–k) plotted as TPM