Fig. 2

Transcriptional modulation of selected antiviral mediators in HK. After 10 weeks of feeding, the fish were injected with vehicle (PBS) or poly(I:C) (2.5 mg/ml) and 24 h later tissues were harvested. The expression of gene transcripts was quantified by qPCR and normalized against the geometric mean of three housekeeping genes (ef1α, drpII, hprt1), and then used for statistical analysis. The transcript expression is reported as arbitrary units and a fold change, calculated as the average expression level of fish injected with poly(I:C) divided by that of fish injected with PBS, is given above the bars. The results represent the mean + SEM from 27 fish from three replicate tanks for each experimental group. The letters above the columns indicate values that are statistically significant versus the controls assessed by one-way ANOVA (p < 0.05), with different letters indicating significant differences between the treatments. The asterisks indicate the transcripts that show a significant interaction between poly(I:C) stimulation and Se supplementation, as assessed by two-way ANOVA