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Table 2 Recovery of flanking sequence tags (FSTs) from Tnt1 insertion mutants NF11217 and NF10547 using TAIL-PCR from different generations

From: Rapid identification of causative insertions underlying Medicago truncatula Tnt1 mutants defective in symbiotic nitrogen fixation from a forward genetic screen by whole genome sequencing

Tnt1 mutant line

Generation

Total FSTs

Novel FSTsa

NF11217

R0

31

31

 

R1

39

30

 

BC1F2

18

10

 

Total

88

71

NF10547

R0

14

14

 

R2

25

24

 

Total

39

38

  1. aInsertion locations of FSTs in the A17 genome from R0 versus R1, and R0 and R1 versus BC1F2 and R0 versus R1 were compared for NF11217 and NF10547, respectively to identify novel, non-redundant FSTs
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BMC Genomics

ISSN: 1471-2164

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