Transcriptomic and metabolomic profiling of ionic liquid stimuli unveils enhanced secondary metabolism in Aspergillus nidulans

Table 5 qRT-PCR analysis of selected genes encoding secondary metabolism components, either biosynthesis or regulation

		Choline		[C₂mim]Cl
GeneID	Gene	qRT-PCR	Microarray^*	qRT-PCR	Microarray^*
Backbone genes
AN7909	orsA	1.67	1.61	2.57	1.35
AN0150	mdpG	−3.62	1.90	−12.50	−1.18
AN8383	ausA	−6.00	−1.30	−3.04	−2.26
AN2547	easB	2.08	2.80	−4.92	−2.67
AN0607	sidC	3.06	4.78	−4.57	−2.28
Transcriptional regulators
AN0807	laeA	−6.75	−6.71	−25.32	−17.55
AN1052	veA	−1.08	−1.23	−1.23	−1.52
AN0363	velB	−1.91	−1.16	1.43	1.45
AN4819	fluG	−1.26	−1.12	−1.89	−1.45
AN4562	rsmA	−1.21	1.12	−1.66	−1.18
AN1905	hepA	−1.06	1.18	1.23	1.70
AN8042	hdaA	1.39	1.04	1.14	−1.18
AN3621	gcnE	1.14	−1.34	1.26	−1.30

Values represent the relative expression of selected genes in pair-wise comparisons with the control, after fifteen days of incubation. Expression of each gene was normalised to the expression of the histone protein H3 gene (AN0733). Corresponding microarray data are shown for comparison
*values highlighted in bold have |FC| ≥ 1.5 and p-value ≤ 0.05 in the microarray data

ISSN: 1471-2164