Fig. 5

Co-immunoprecipitation of MUPP1 with CaMKII in mouse spinal cord tissue. Spinal cord synaptosomes were first incubated with anti-MUPP1 (or IgG as a negative control). After column purification, the immune complexes were eluted and separated on acrylamide gels. Blots were probed with anti-CaMKII to quantify MUPP1-CaMKII association. Panel (a) provides the results of co-immunoprecipitation experiments demonstrating that MUPP1-CaMKII association is unchanged by morphine treatment in C57BL/6 mice. In panel (b) the results suggest MUPP1 protein from 129S1/Svlm and C57BL/6 mice bind CaMKII similarly. For each measurement the mean value is displayed +/− S.E.M., n = 6 mice per group