Fig. 5
Identification of 20 ERF subfamily genes in two cassava cultivars (‘KU50’ and ‘SC5’) under water-deficit stress. a Heat map of real-time quantitative PCR (qRT-PCR) results for 20 ERF subfamily genes in two water-deficit stressed cassava cultivars; three biological and technical replicates were performed. b The water-deficit stress phenotype of two cassava cultivars, ‘KU50’ and ‘SC5’, at the middle stage of leaf abscission. Real-time PCR primer sequences are listed in Additional file 7: Table S7. The expression ratios for each ERF gene at each time point are presented first using T1 as a control for both ‘KU50’ and ‘SC5’, and then to cut ‘KU50’ expression ratios with those of ‘SC5’ at each time point. The heat map depicts the log2-transformed fold change values. The log2 (fold change values) and the colour scale are indicated above the heat map