Fig. 3

X-linked gene expression during the first wave of spermatogenesis. a Boxplots of the difference from mean gene expression at 8 dpp are shown for each time point, with genes on the autosomes in purple and those on the XY chromosomes in green. Gene expression is log2(TPM + 1). b Heatmap of X-linked gene expression at each time point. Genes are clustered based on the pattern of expression: an increase of expression at 12 dpp and then stable throughout (Stable), steadily decreasing over time (Decreasing), or low expression from 8-10 dpp and high expression from 12-14 dpp followed by low expression at 16-18 dpp (Transient). Gene expression is shown as average log2(TPM + 1) over time-point replicates