Table 1 Comparison of total number of junctions identified by RSR for five cases from Tg and Dtt treated samples
Parameter | Case 1 | Case 2 | Case 3 | Case 4 | Case 5 | |
|---|---|---|---|---|---|---|
Variable Name | Minimum split size | 8 | 11 | 11 | 15 | 18 |
Maximum candidate distance | 40,000 | 40,000 | 50,000 | 50,000 | 50,000 | |
Read mapping region boundary buffer size | 5 | 5 | 5 | 8 | 8 | |
Minimum candidate distance | 2 | 2 | 2 | 2 | 2 | |
Minimum number of supporting reads | 2 | 2 | 2 | 2 | 2 | |
Maximum good alignment allowed per read | 8 | 11 | 11 | 15 | 18 | |
Tg Het | Total number of junctions identified | 122 | 140 | 143 | 135 | 141 |
Tg KO | Total number of junctions identified | 153 | 177 | 177 | 170 | 177 |
Dtt Het | Total number of junctions identified | 6496 | 6614 | 6661 | 6673 | 5683 |
Dtt KO | Total number of junctions identified (Novel/Known) | 6135 | 6247 | 6285 | 6308 | 5687 |