Fig. 1

RNA-seq highlights expression differences between ovary and VG tissue. a Principal component analysis of expression profiles for ovary (green) and VG (blue) replicates showing the samples clustering by treatment group. The percentage on the axis labels represents the total variance explained by that component (b) Volcano plot of the RNA-seq data with positive fold change representing up regulated genes in the VG and vice versa for ovary. Genes coloured as red were differentially expressed at an adjusted p-value < 0.05. c Pie chart summarising the proportion of genes that were part of each expression category (adjusted p-value < 0.05). d Semi-quantitative RT-PCR targeting the highest expressed annotated venom encoding gene Nasvi2EG007167. Amplification of Nasvi2EG007167 cDNA could be detected at 24 cycles in the venom gland cDNA replicates, whilst only a faint band could be detected at 30 cycles for the ovary samples. The house-keeping gene RP49 was used as a loading control. VG1-3 and O1-3 represent independent replicates for venom gland and ovary samples, respectively