Fig. 3

Nutrient starvation and ER stress induce a common transcriptional landscape in murine enteroids. a Venn diagram comparison of the genes commonly upregulated more than 2.5 fold upon nutrient starvation and thapsigargin treatment. Hypergeometric tests were used to calculate the P values for significance of overlaps. Scatterplot analysis of the genes transcriptionally upregulated (cutoff >2.5 fold) upon thapsigargin and nutrient starvation. Plots are displayed analyzing of the total number of genes (801) (b) or categorized by gene function (c). d Analysis of the gene function of the 90 genes commonly upregulated upon thapsigargin and nutrient starvation. e Expression profiles of genes encoding transcription factors and genes involved in inflammation/chemotaxis, manually picked from the 90 genes upregulated more than 2.5 fold upon thapsigargin/nutrient starvation. Expression profiles of three biological replicates per stimulation are shown (lanes a, b and c)