Fig. 4

Sequence GC content on two var regulatory regions is associated with their chromatin accessibility. a Scatterplot shows the correlation between sequence GC content and chromatin accessibility of genome 1 kb bins during ring and trophozoite stages (see Methods). Each point represents a 1 kb bin in P. falciparum genome. X-axis and Y-axis represent the correlation value between GC content of each 1 kb bin (each bin is divided again into 10 bins with length 100 bp) and its FAIRE-Seq signal during ring and trophozoite stages. Green histogram plot represents the distribution of correlation value. b Average sequence GC content around var intron and 2 kb upstream regions is plotted. c The sequence GC content distribution of different genome regions are plotted (‘C_2kb’ and ‘C_intron’ indicate 2 kb upstream and intron region of P. falciparum genes with one intron, ‘*’ represents Wilcoxon-Rank-Sum test p-value < 0.05). d The bee swarm plot shows Pearson correlation between sequence GC content of different genome regions and their FAIRE-Seq signal during different blood stages (‘*’ represents Wilcoxon-Rank-Sum test p-value < 0.05 compared with other elements)