Table 1 Assembly and annotation of Spartina alterniflora transcriptome

Number of raw reads

770,690

Number of reads in leaf tissue

459,819

Number of reads in root tissue

310,871

Total span of reads

249.59 Mbp

Average length of reads

~324 bp

Number of Unigenes (50 bp or greater)

273,460

Number of Contigs (50 bp or greater)

73,131

Large Contigs (500 bp or greater)

35,519

Large Unigenes(500 bp or greater)

39,682

Number of Singletons (50 bp or greater)

200,329

Number of Unigenes (Leaf Control)

73,552

Number of Unigenes(Leaf Stress)

68,346

Number of Unigenes (Root Control)

72,199

Number of Unigenes (Root Stress)

59,363

Total span of Contigs

42.19 Mbp

Total Span of Singletons

63.29 Mbp

Transcriptome Size

105.48 Mbp

N50 length

52.74 Mbp

N50 Value

317 bp

N90 length

94.93 Mbp

N90 Value

255 bp

Longest Unigene

6.46 Kb

Smallest Unigene

51 bp

Mean length of Unigenes

~386 bp

GC content (%)

51.46

Unigenes with significant hit (%)

187,571 (68.59 %)

Unigenes with unknown functions

9,684 (5.16 %)

Unigenes having unique protein annotation

47,960 (25.56 %)