Fig. 1

Schematic flowchart of the G23D pipeline and the resources being used. Genomic coordinates are converted to protein coordinates using dbNSFP. The proteins are then used to retrieve models from ModBase and their sequence is used for a blast search against the PDB. S2C (Roland Dunbrack lab) is used to convert sequence position to index of coordinates within PDB files. JSmol is used to visualize the hits. Side chain modeling programs are applied to model the mutant side chain. Comparative contact analyses using contact surface areas and stability analyses are based on the structure hits. Shaded boxes indicate steps which are pre-processed in advance and not during run-time