Fig. 3From: Distinct gene expression program dynamics during erythropoiesis from human induced pluripotent stem cells compared with adult and cord blood progenitorsThe gene expression profile of hiPSC derived erythroblasts is independent of the media used as evidenced by comparison of erythroid differentiation in SEM-i with SEM-F. a Hierarchical clustering analysis by Euclidean distance of adult erythroid differentiations performed using media for adult derived HSPCs (SEM-F), or hiPSC conditions (SEM-i). The gene set used contained genes which were DE during maturation in any of the two media settings (Additional file 20: Table S4). The colour bar on the left hand side denotes clusters of co-regulated genes. Samples cluster together by time and by the immunophenotype of the developing erythroid cells. There are no coherent subclusters formed according to the type of media used. b PCA of the samples shown in (A) with the same gene set. Populations are represented as follows: SEM-F, red symbols; SEM-i, blue symbols; d0 CD34+, black circles; day 4 CD36+CD71+CD235a−, triangles; day 7 CD36+CD71+CD235a−, thin diamond; day 7 CD36+CD71+CD235a+, square; day 7 CD71+ beads, fat diamond; day 14 CD71+CD235a+, pentagon; day 14 CD235a+ beads, hexagon. c Proliferation in SEM-i of erythroid AB-erythroblasts (black triangles), CB-erythroblasts (green squares) and hiPSC-erythroblasts (blue) where hiPSCs were specified from CD34+ peripheral blood (squares), erythroid cells (triangles) or fibroblasts (circles). Error bars indicate standard error of the mean of 3 or more cultures. d Morphological changes observed in erythroblasts of hiPSC and AB origin cultured in SEM-i. Representative images of Giemsa-benzidine stained cytospins of cultures on day 7, day 14 and day 20. Scale bar is ~10 μm. “m” is the stromal cell line MS-5. AB-derived differentiations, cultured further until day 20/21, were typically 70-80 % enucleated (see also Additional file 21: Figure S12), whereas the hiPSC-erythroblast cultures failed to enucleateBack to article page