Fig. 8
qRT-PCR validation of the differently expressed genes. Fifteen up-regulated genes and thirteen down-regulated genes were identified using qRT-PCR. The 15 up-regulated genes included three esterase genes (unigene13173, unigene29498, and unigene15941), two cytochrome P450 genes (unigene17631 and unigene12321), three hemolin genes (unigene12340, unigene586, and unigene1173), two twitchin genes (unigene12898 and unigene3568), two heat shock protein genes (unigene1911 and unigene3432), two tropomyosin genes (unigene25476 and unigene4859) and one chemosensory protein gene (unigene3898). The 13 down-regulated genes included three cuticular protein genes (unigene3900, unigene4018, and unigene1448), three protease genes (unigene2960, unigene6059, and unigene7750), four oxidoreductase genes (unigene5356, unigene5215, unigene6772, and unigene2729), one neuronal pentraxin gene (unigene249), one glutathione S-transferase gene (unigene10933) and one cytochrome P450 gene (unigene 2468). The left y-axis indicates the relative expression levels based on qRT-PCR, and the right y-axis indicates the log2 ratio of F-treated/Control based on DGE analysis. Error bars indicate the average deviations of the three replicates