Fig. 5

Transrate assembly scores and reference coverage metric. a Compares the Transrate [13] assembly score and the optimised score between the CDS and PASAua [27] datasets in the four species (Ddis: D. discoideum, Ppal: P. pallidum, Dfas: D. fasciculatum, Dlac: D. lacteum). The dotted lines represent the Transrate scores that would be better than 50% of 155 published de novo transcriptomes as found by Smith-Unna and co-workers [13]: 0.22 overall score (black horizontal dotted line) and 0.35 optimal score (cyan horizontal dotted line). b The proportion of reference protein sequences covered by transcripts in the CDS and PASAua datasets by at least 25%, 50%, 75%, 85% and 95% of the reference sequence length