Fig. 2

RT-PCR supports RNA-seq-predicted transcripts. RNA was isolated from haploid cells (518, 521), dikaryotic mycelia (UmDIK), dormant teliospores (T00), and U. maydis-infected seedlings at 8, or 14dpi and used as template for RT-PCR. Oligo-(dT)₁₆, DEPC-treated water or a strand-specific primer was used to prime the reverse transcription reaction. Only the transcript-specific reaction is shown above, along with a genomic DNA PCR control (gDNA) and a no template control (NTC). A size marker was also included (M)