Fig. 2

The Maxima H Minus reverse transcriptase provides higher yield of cDNA and quality of libraries. a cDNA yield assessment. X-axis indicates various UHR RNA input amounts used for mRNA isolation and cDNA synthesis. Double strand cDNA was measured using the Qubit HS DNA assay. Values from this assay were normalized relative to the value obtained when using Superscript II (RT-II) for the 250 ng input. b Diversity of libraries. Libraries were generated from cDNA samples that were prepared using the best performing RT (Maxima) and Superscript II (SS-II). The resulting sequencing data were analyzed for duplicate rates. c ERCC spike-in sequence differences. Mismatch rates were calculated by comparing observed sequences and expected sequences from the known spike-in synthetic RNAs. X-axis represents various UHR RNA input amounts used for mRNA isolation and cDNA synthesis. Y-axis is error rate per 1000 nucleotides. n = 3; error bars = Standard Deviation. *P < 0.05. P values were calculated using Student’s t-test (unpaired and equal variance)