Table 1 Sample information
ID | Age | Sex | RNA Yield (ng/ml) | PAXgene yield (% Tempus) | RIN | ||
|---|---|---|---|---|---|---|---|
PAXgene | Tempus | PAXgene | Tempus | ||||
Normal controls in PAXgene and Tempus tubes for RNA extraction | |||||||
 Control-1 | 73 | M | 5510 | 6572 | 84 | 7.0 | 7.5 |
 Control-2 | 33 | M | 1082 | 1339 | 81 | 8.0 | 7.9 |
 Control-3 | 37 | M | 3262 | 3598 | 91 | 7.4 | 7.7 |
 Control-4 | 51 | M | 3716 | 2279 | 163 | 7.1 | 9.1 |
 Control-5 | 26 | M | 3826 | 3324 | 115 | 7.8 | 8.8 |
 Control-6 | 63 | M | 1071 | 2636 | 41 | 7.7 | 7.6 |
 Control-7 | 32 | M | 1007 | 1858 | 54 | 7.8 | 9.5 |
 Control-8 | 30 | M | 3676 | 3930 | 94 | 7.0 | 8.5 |
 Control-9 | 40 | F | 4669 | 5772 | 81 | 7.7 | 7.5 |
TrialNet type 1 diabetes patient samples in Tempus tubes | |||||||
ID | Age | Sex | Duration (y) | Serum Auto-antibodiesa | RIN | ||
 TN-T1D1 | 23 | M | 2.25 | GAD65, ICA512, mIAA, ICA | 7.4 | ||
 TN-T1D2 | 23 | F | 2.25 | GAD65, ICA512, mIAA | 8.4 | ||
 TN-T1D3 | 26 | F | 2.28 | GAD65, mIAA | 7.8 | ||
 TN-T1D4 | 13 | F | 2.26 | ICA512, mIAA, ICA | 8.5 | ||
 TN-T1D5 | 40 | M | 2.22 | GAD65, ICA512, mIAA | 8.6 | ||
 TN-T1D6 | 18 | F | 2.26 | GAD65, mIAA | 8.2 | ||
 TN-T1D7 | 18 | M | 2.24 | GAD65, ICA512, mIAA | 7.6 | ||
 TN-T1D8 | 14 | F | 2.24 | GAD65, mIAA | 7.6 | ||
 TN-T1D9 | 16 | F | 2.19 | ICA512, mIAA, ICA | 7.3 | ||
 TN-T1D10 | 16 | M | 2.28 | GAD65, ICA512, mIAA | 8.3 | ||
University of Florida type 1 diabetes patient samples in PAXgene tubes | |||||||
ID | Age | Sex | Duration (y) | Serum Auto-antibodiesa | RIN | ||
 UF-T1D1 | 17 | F | 2.67 | GADA, IA-2 | 9.7 | ||
 UF-T1D2 | 16 | M | 2.42 | Not detected | 7.1 | ||
 UF-T1D3 | 16 | M | 2.00 | GADA, IA-2, ZnT8 | 9.1 | ||
 UF-T1D4 | 19 | F | 2.33 | GADA | 7.9 | ||
 UF-T1D5 | 17 | F | 2.25 | GADA, IA-2, ZnT8 | 8.8 | ||
 UF-T1D6 | 27 | M | 1.92 | GADA, IA-2, ZnT8 | 8.0 | ||
Normal controls for RNA extraction and lysate preparation | |||||||
ID | Age | Sex | RNA Yield (ng/ml) | PAXgene yield (% Tempus) | RIN | ||
PAXgene | Tempus | PAXgene | Tempus | ||||
 Sample 1 | 19 | M | 1508 | 4690 | 32 | 6.3 | 9.0 |
 Sample 2 | 19 | M | 947 | 3088 | 31 | 8.4 | 8.8 |
 Sample 3 | 20 | F | 1441 | 4897 | 29 | 8.6 | 8.0 |
 Sample 4 | 19 | F | 2982 | 3383 | 88 | 8.7 | 8.6 |
 Sample 5 | 19 | M | 2604 | 3249 | 80 | 9.2 | 8.6 |
 Sample 6 | 24 | M | 2016 | 905 | 223 | 8.2 | 8.7 |
 Sample 7 | 20 | F | 2112 | 2942 | 72 | 9.2 | 7.0 |
 Sample 8 | 24 | F | 3218 | 3769 | 85 | 8.5 | 8.2 |
 Sample 9 | 19 | M | 1013 | 1916 | 53 | 8.2 | 8.7 |
 Sample 10 | 29 | F | 3411 | 4031 | 85 | 8.2 | 8.7 |
 Sample 11 | 26 | M | 5129 | 3923 | 131 | 7.5 | 9.0 |