Table 2 Top 30 significantly DE miRNAs and their predicted target genes from microarray analysis in PMECs at 24 hpc with E. coli compared with unchallenged controls

Upregulated miRNA

Sequence (5′ - > 3′)

Downregulated target genes

 miR-505

CGUCAACACUUGCUGGUUUCCU

ABCA7, DDX18, DDX52, EIF4E, GRPEL1, LARP4, MSL1, PLEKHH1, SDC1, SERTAD1, SOCS4, TATDN2, WDR3, YTHDF2, ZBED4

 miR-29a

UAGCACCAUUUGAAAUCGGUU

CDC6, CYP26A1, DNAJA1, EML5, RNF2, TIMM44

 miR-101

UACAGUACUGUGAUAACUGAAG

CHD7, EML5, HELLS, LARS, MID1, RBM12

 miR-24-star

GUGCCUACUGAGCUGAUAUCAGU

USP46

 miR-289

UAAAUAUUUAAGUGGAGCCUGCGACU

KIAA1430, RNF2, TMCC3

 miR-125-star

ACGGGUUAGGUUCUUGGGAGC

GIT2, LARS, MSL1, PAFAH1B1, RBM12, SDC1, TMEM79, UBE2R2, ULK3, USP46

 miR-484

UCAGGCUCAGUCCCCUCCCGAU

FGFR3

 miR-22-star

AGUUCUUCAGUGGCAAGCUUUA

ARGLU1

 miR-660

UACCCAUUGCAUAUCGGAGCUG

ARMC8, BCLAF1, CAMKK2, CPT1A, DDX42, MSL1, NOL6, NRARP, PLEKHH1, PPP1R8, PPP6C, PSMD12, PSMF1, PYGO2, RRP12, SDC1, TRIM27, ZFX, ZNF280D

 miR-B6

AAGUGCCCGACGCGGGGAACGUG

ENC1

 miR-363

AAUUGCACGGUAUCCAUCUGUA

ABCA7, ADPRH, ATXN1, CAMKK2, DGCR8, FAM53C, GABPB1, GATAD2A, HERPUD2, PAFAH1B1, PLEKHH1, POP4, PPRC1, RBM15, S1PR2, SYK, TATDN2, TFAP2C, TRIM35, TXLNA

 miR-128

UCACAGUGAACCGGUCUCUUUU

ADPRH, AQP3, GABPB1, KCNJ14, NDNL2, PLEKHH1, RRP12, SDC1, SNX6, TCEB3, UBE2R2, ULK3, ZFAND2A, ZFX, ZNF764

 miR-10a

UACCCUGUAGAUCCGAAUUUGUG

ADPRHL2, EML5, FARSA, FEN1, IQCC, KLHL18, LMNB2, MFSD9, SFN, SLC16A3, SLC39A6, TATDN2, TLE1, TMCC3, TUBB2B, TXLNA, UBAP2L, ZNF367

 miR-92b

AAUUGCACUAGUCCCGGCCUGC

CPT1A, GPRC5A, HEXIM1, LIN37, PHF23, PKP1, ULK3, YWHAG

 miR-92b

UAUUGCACUCGUCCCGGCCUCC

EML5

Downregulated miRNA

Sequence (5′ - > 3′)

Upregulated target genes

 miR-371

ACUCAAAAAAUGGCGGCACUUU

SGSM1

 miR-4423-3p

AUAGGCACCAAAAAGCAACAA

RASAL2

 miR5019

UGUUGGGAAAGAAAAACUCUU

RASAL2

 miR-3128

UCUGGCAAGUAAAAAACUCUCAU

SLC2A12

 miR5021

UGAGAAGAAGAAGAAGAAAA

CIRBP

 miR-M9-3p

AAACUCCGAGGGCAGGAAAAAG

ARHGAP12, ARHGAP28, BIRC3, CEP68, FCHSD2, GRK5, ISG15, LPIN1, MDM4, PCDH7, PDLIM5, SLC2A12, TCTN2, YPEL2

 miR3949

UGAUGUUGAGGCAAAAAUGUAG

CCDC152, RBM39, RFX1,

 miR-B8-5p

CGCGGGCAAAAAAUCCAAUGGC

BIRC3, MAP3K8, PLXND1, RNF19B

 miR-2326

CCCCCCUUCCUCUGGAAAAA

ARHGAP28, CEP68, CHI3L1, CXCL2, FNBP1, LPIN1, PCDH7, PLXND1, PPAP2B, RFFL, RHOQ, SCUBE3, SLC4A7, TPM2

 miR-5100

UCGAAUCCCAGCGGUGCCUCU

OBFC1

 miR-259-star

CCACCGAUUUGGCAUGGGAUUGAC

SGSM1

 miR-2779

AUAUCCGGCUCGAAGGACCA

CLDN1, PLXND1

 miR-4592

CCAGCGGCGGUGCCGUGAUGGCGA

CLDN1, CEP68, CXCL2, ITGB8, MDM4, RHOQ

 miR-202

AGAGGCAUAGAGCAUGGGAAAA

ARHGAP28, BIRC3, CHL1, GLI3, LIFR, MAP3K8, P2RX4, PLN, RASAL2, RFFL, RMND5A, RNF19B, SGSM1, TEP1, TNFAIP3, TPM2, VPS37C

 miR-3277

UGGGCAUGUUUCUGAAAUUCGAU

ARHGAP28, IFNAR2, IL1A, PPARG