Fig. 1

Dissection, PCA and clustering of samples in B. mori oogenesis. a Dissection of sequenced samples in a single ovariole of the day 7 pupa. The central elliptical figure shows the single ovary of the day 7 pupa containing four ovarioles. Each ovariole was divided into 15 samples, based on the transition point from vitellogenesis to choriogenesis, represented by a short red line. The samples of previtellogenesis and vitellogenesis are marked in yellow. The choriogenesis samples are marked in green. The microstructures at Time 1, Time 3, Time 8, Time 9, Time 11 and Time 15 were obtained. O and NC represent oocyte and nurse cells, respectively. FC represents follicular cells. CM represents chorion membrane. All microstructures were scaled to 100 μm. b Principal component analysis results of total 15 samples based on the expression profiles. Different colors indicate various groups. c Clustering dendrogram of samples based on their average Euclidean distance