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Fig. 4 | BMC Genomics

Fig. 4

From: Trypanosoma cruzi specific mRNA amplification by in vitro transcription improves parasite transcriptomics in host-parasite RNA mixtures

Fig. 4

Performance of T7SL IVT on RNA mixtures. a Length distribution profiles for aRNA produced from pure samples (T. cruzi epimastigote and HeLa), mixture of T. cruzi (epimastigote) and HeLa and blank samples (no RNA for amplification reaction). Percentage are relative mass of Epi:HeLa on RNA mixture used for T7SL IVT. b After T7SL IVT and RNA-Seq of mixture samples, reads were aligned to T. cruzi and human genomes. The percentage of aligned reads with best match on parasite or human genome were retrieved and plotted. The input mass (in ng) used in each mixture is specified at the bottom. c Scatterplot comparing T7SL Pfx IVT and mixtures of HeLA-Epi RNAs, or PolyA+ RNA against HeLA-Epi RNAs. d Scatter plots of epimastigote to trypomastigote fold changes when comparing PolyA(+) transcriptome quantification to T7SL using T.cruzi/HeLa RNA mixtures

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BMC Genomics

ISSN: 1471-2164

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