Fig. 1

Experimental approach to identify eyespot associated genes, sector-specific transcription factors (TFs), and sex-biased eyespot gene expression. a Micro-dissected tissues were collected from two wing sectors, M3 and Cu1 during the early pupal stage. b The Spotty mutation adds two additional eyespots to the wing, one in sector M3. c Genes associated with eyespot development were identified as the set of genes that were differentially expressed between wing sectors that do not develop eyespots versus wing sectors that develop eyespots across three comparisons between homologous and non-homologous wing sectors of wild-type (Wt) and Spotty (Sp) wings. d Wing sector specific TFs were identified as the set of common differentially expressed genes between wing sectors M3 and Cu1 in both Wt and Sp wings. e Sexually dimorphic transcripts were identified as differentially expressed genes between Cu1 male (Wt) and Cu1 female (Wt) wing sectors