Fig. 5

circRNA levels increase in aging photoreceptors. a Schematic showing how junction-spanning reads were used to detect circRNAs resulting from back-splicing events. b Volcano plot showing the fold change in circRNA abundance plotted as log₂(fold change in counts per million reads, CPM) for each circRNA relative to its p value (−log₂[p.value]). CircRNAs with significantly differential expression (p ≤ 0.05 and FC ≥ 2, dotted lines) are highlighted. Labels indicate the corresponding host gene for selected circRNAs. c Fold changes in circRNA abundance for pairwise comparisons between the indicated aging time-point. CircRNAs with significantly differential expression (p ≤ 0.05 and FC ≥ 2, blue/red) are highlighted. d Total abundance of circRNAs (CPM) identified at each age. p values, non-parametrical Kruskal-Wallis with Nemenyi post-hoc test for multiple comparisons. e Density plots comparing the log₂ fold changes in circRNA CPM with fold change in linear RNA RPKM from the corresponding gene for 10 versus 40 day sensory neurons. f Linear regression analysis of the mean CPM of the 35 significantly upregulated circRNAs versus age