Table 3 Relative gene expression determined by real time RT-qPCR (2-∆∆Ct). Gene expression was normalized to the expression of Ef1α and is presented as mean value of the Lso + treated plants compared to the Lso- treated plants for each variety
GENE ID | Gene function | Mean 2-△△Ct value in Atlantic | Mean 2-△△Ct value in Waneta |
|---|---|---|---|
Genes down-regulated in Atlantic and Waneta after Lso treatment | |||
PGSC0003DMG400012822 | Stem-specific protein TSJT1 | 0.0291977 | 0.1709327 |
PGSC0003DMG400004301 | Chlorophyll a,b binding protein type I | 0.0536232 | 0.0844505 |
PGSC0003DMG400033084 | Chlorophyll a/b-binding protein (cab-12) | 0.1027244 | 0.0373299 |
PGSC0003DMG400009869 | DNAJ | 0.2373127 | 0.0143763 |
PGSC0003DMG400011751 | 2-oxoglutarate-dependent dioxygenase | 0.02778 | 0.134926 |
PGSC0003DMG400012763 | C-4 sterol methyl oxidase | 0.0117969 | 0.0495508 |
Genes up-regulated in Atlantic and down-regulated in Waneta after Lso treatment | |||
PGSC0003DMG400006179 | Nodulin family protein | 9.564544 | 0.1504254 |
PGSC0003DMG400036566 | Ethylene response factor 5 | 2.2506224 | 0.1328974 |
PGSC0003DMG400031457 | Phenylalanine ammonia-lyase 1 | 11.298391 | 0.036989 |
PGSC0003DMG402007970 | Conserved gene unknown function | 30.013692 | 2.2496471 |
Genes regulated in Atlantic | |||
PGSC0003DMG400011740 | SGA | 0.0212225 | |
PGSC0003DMG400027453 | Ribonuclease t2 | 0.1067257 | |
PGSC0003DMG400009513 | Aspartic protease inhibitor 5 | 0.1006475 | |
PGSC0003DMG400032792 | Calmodulin-binding protein | 13.664482 | |
PGSC0003DMG400011633 | WRKY-type transcription factor | 4.8602585 | |
Genes regulated in Waneta | |||
PGSC0003DMG400000332 | Salt responsive protein 2 | 0.0393988 | |
PGSC0003DMG400010713 | Salt responsive protein 2 | 0.0777253 | |
PGSC0003DMG400019964 | Conserved gene unknown function | 0.3503861 | |
PGSC0003DMG400025721 | Conserved gene f unknown function | 7.2729239 | |