A next generation sequencing based approach to identify extracellular vesicle mediated mRNA transfers between cells

Table 2 Number of genes involved in the transfer from adipocytes to macrophages, those possibly mediated by exosomes, and their overlap significance

	# transcripts measured in ADMO_exosome	# Sig Gene^a	FPKM > 0			FPKM > 1
	# transcripts measured in ADMO_exosome	# Sig Gene^a	All^b	overlap	p-value^c	all	overlap	p-value
AD to MO	60,566	20	13,697	17	<2.2E-16	1966	5	< 2.2E-16
MO to AD	60,566	8	13,697	8	<2.2E-16	1966	2	< 2.2E-16

^aThe significant gene is defined at FDR < = 0.05
^bAll (overlap) denotes the number of transcripts with FPKM > 0 (and overlapping with significant genes) for ADMOexosome
^cThe p-value is obtained by testing the overlapping significance between “All” and “Sig Gene” using the Fisher’s exact test with all 60,566 transcripts measured as background

ISSN: 1471-2164