Fig. 2

CBP knockdown causes lethality in larvae, pupae and adults of Tribolium. dsCBP was injected into newly molted last instar larvae, newly formed pupae and newly emerged adults. Phenotypes observed after 4 days after dsCBP injection into larvae (a), pupae (b) and adults (c) respectively. Dissected alimentary canals (mostly midgut region) from dsmalE and dsCBP injected larvae (d), pupae (e) and adults (f). Arrows point to the enlarged areas of the alimentary canals that showed darkened regions. Knockdown efficiency at 24 h after dsCBP injection in larvae (g), pupae (h) and adults (i). Percent mortality observed at 8 days after dsCBP injection into larvae (j), pupae (k) and adults (l). Scale Bar: 1 mm. [One way AVOVA, * P < 0.05]