Fig. 5

Promoter strength. Results of the Random Forest (RF) computation and analysis of the variation of promoter consensus across various transcription levels in B. breve UCC2003. a Barplot showing the results of the RF analysis conducted on 62 bp regions upstream of predicted TSSs. The plot shows the importance of each base position across the 62 bp region where the promoter elements (−10 and −35) can be detected (red blocks). Importance values of a number of additional classifiers is also indicated (green block). b Weblogo showing the consensus motif identified for the −10 and −35 sequences of the canonical promoter in B. breve across the levels of expression high, medium and low. Also in this case the promoter elements (−10 and −35) can be detected