Fig. 3

Expression of the PttSCAMP genes in the wild type and the three PttSCAMP3 RNAi lines. a Relative expression (as a percentage of the WT) is shown for differentiating xylem samples scraped from the base of two-month-old trees. The expression is based on gene expression values in the RNA sequencing datasets, where read counts were normalized for the RNAseq library size. Asterisks indicate significant difference from the wild type at P(Benjamini-Hochberg adjusted) < 0.05 according to the R-package DESeq2. Vertical bars indicate ± SD. n = 5. b Relative expression of PttSCAMP3 (as a percentage of the WT) by qPCR analysis. Asterisks indicate significant difference from the wild type at P < 0.05 (*) or P < 0.001 (***) according to Welch corrected t-test. Vertical bars indicate ± SD. Three biological replicates were analyzed in three technical replicates each