Fig. 11

FuSpot Visualization of VDAC1-VDAC1P2 False Positive Fusion Junction Reported by Bellerophontes. FuSpot’s visualization of the putative VDAC1-VDAC1P2 fusion gene claimed by Bellerophontes when given the BEERS data set [25]. Each read seen here was extracted from either the normal or chimeric output file produced by aligning the BEERS [25] data set with STAR. The reads in Group 1 and 3 align as non-fusion reads to their respective gene partners. However, FuSpot’s realignment and visualization exposes the reads in Group 2 which all lack biological significance. Similar to the THRB-AZIN2 fusion point in Fig. 8, these alignments show that the VDAC1 and VDAC1P2 sequences on the 3′ side of the breakpoint are highly similar and is likely what caused the tool to report the fusion candidate. We can visually reject this candidate from downstream analysis