Fig. 3
From: JAK/STAT3 regulated global gene expression dynamics during late-stage reprogramming process
JAK/STAT3 Signaling Controls the Activation of Key Genes for Germ Cell Development but Not Initial MET Transition in Reprogramming. a Pie charts for summarized GO-terms using DEGs downregulated between Ctl and Jaki treatments at S2. The number of DEGs with GO terms vs. the total number of DEGs was shown under the chart. b Venn Diagrams for DEGs from specific GO-terms upregulated from S1 to S2 under Ctl reprogramming condition but downregulated at S2 by Jaki-treatment compared with the Ctl. Upper left: DEGs from meiotic GO-terms, upper right: DEGs from mitotic GO-terms, bottom: DEGs from DNA damage/repair GO-terms. c Number of genes detected exclusively under either DMSO or Jaki treatment at S1 and S2 and their relevant GO-terms (FDR < 0.1). d qPCR analysis for MET markers to reprogrammed cells collected at two different reprogramming conditions and stages, with the expression in non-reprogrammed OG-MEFs set as the control. Bars represent mean ± SD from three independent biological repeats. **: p < 0.01. e Heatmap of FPKM values of core epithelial/mesenchymal marker genes inreprogrammed cells at two different stages and conditions. The relative abundance is represented by color (blue, lower abundance; red, higher abundance), as indicated by the color key