Table 3 Validated allelic variants in detected QTLs. Selected target genes for the validation of QTLs influencing fermentation kinetics, substrate consumption and the production of fermentative aromas; differences caused by the allelic gene variants regarding the influenced traits were detected by RHA and are given as the ratio of phenotype MTF2621 to phenotype MTF2622
QTL name | Trait | Evaluated genes | Different impact of allele on trait as MTF2621/MTF2622 [factor] |
|---|---|---|---|
chr2@166.4 | Ethyl butanoate Ethyl lactate Pyruvate yield | AGP2 | 0.79*** ethyl lactate |
chr3@26.1 | 2-methylpropanoic acid | AGP1 | 1.26* 2-methylpropanoic acid |
ILV6 | no effect | ||
chr4@71.3 | Ethyl 2-methylbutanoate Ethyl butanoate Ethyl 2-methylpropanoate Pyruvate yield | YDL124W | no effect |
chr4@133.6 | 2-methylbutyl acetate 3-methylbutyl acetate Ethyl hexanoate Methionol | SIR2 | 0.77* 3-methylbutyl acetate 0.78** ethyl hexanoate |
chr4@177.5 | Ethyl lactate Ethyl octanoate Diethyl succinate Propanoic acid Propanol Propyl acetate | NRG1 | 1.10* propanol |
chr4@386.5 | CO2 production rate at t80% | HXT3 | 1.07* CO2 production rate |
| Â | G/F ratio | Â | 1.86** G/F ratio |
| Â | Â | HXT6 | no effect |
| Â | Â | HXT7 | no effect |
chr11@29.5 | 2-phenylethyl acetate Ethyl decanoate Ethyl dodecanoate Ethyl hexanoate Ethyl octanoate Decanoic acid Hexanoic acid Octanoic acid t80% | ACP1 | no effect |
FAS1 | 0.81** ethyl hexanoate 0.82** decanoic acid 0.84**hexanoic acid 0.89** octanoic acid | ||
FAT3 | no effect | ||
PXA2 | no effect | ||
chr11@127.6 | 2-methylpropanoic acid 2-methylpropanol 2-methylpropyl acetate 3-methylbutanoic acid 3-methylbutanol | IXR1 | 1.14** 2-methylpropanol 1.16* 2-methylpropanoic acid |
MAE1 | 1.43** 2-methylpropanoic acid 1.67*** 2-methylpropanol 1.27*** 3-methylbutanoic acid 1.40* 3-methylbutanol | ||
RGT1 | 1.15*** 2-methylpropanol | ||
chr12@226.9 | Propanol Propyl acetate | whole region | no effect |
chr14@48.9 | Dodecanoic acid Propanoic acid Propanol Propyl acetate Valeric acid | ALP1 | 0.90* dodecanoic acid 1.07** propanol 1.26*** valeric acid |
chr15@176.5 | 2-methylbutyl acetate 2-phenylethyl acetate 3-methylbutanoic acid | LEU9 | 1.08* 2-phenylethyl acetate |
RGS2 | 1.21* 2-methylbutyl acetate 0.83* 2-phenylethyl acetate |