Identification of novel putative causative genes and genetic marker for male sterility in Japanese cedar (Cryptomeria japonica D.Don)

Table 2 Development of SNaPshot primer set

Locus position	Forward/Reverse	Primer sequences	Amplicon size (bp)	Forward/Reverse	Extension primers	No.of nucleotides (bp)	Alleles (dyes) detected
Locus position	Forward/Reverse	Primer sequences	Amplicon size (bp)	(Sense/Anti-sense)	Extension primers	No.of nucleotides (bp)	Alleles (dyes) detected
reCj19250_1927	Forward	5’-CTGGAAAGGTCATGTACTCTGC-3’	190	Forward	5′-1 T-AACATATGCGTTCACTACCCTGC-3’	24	G (blue), A (green)
reCj19250_1927	Reverse	5’-TCCCATCTACAGTAAGAGACATCC-3’	190	(Sense)	5′-1 T-AACATATGCGTTCACTACCCTGC-3’	24	G (blue), A (green)
reCj19250_2335	Forward	5’-GTTCTAGACGTGGCGGCAG-3’	180	Reverse	5’-TGCTCAATCCTGTACACAGTACCTGCAC-3’	28	C (yellow), T (Red)
reCj19250_2335	Reverse	5’-ACCTCGGAATGAACATTATGTGTTGAG-3’	180	(Anti-sense)	5’-TGCTCAATCCTGTACACAGTACCTGCAC-3’	28	C (yellow), T (Red)
reCj19314_126	Forward	5’-CGCTCTTCGTGTTGGCTTAG-3’	133	Reverse	5′-7 T-TCTCTGTGTGCCGACGAAACCGAAGT-3’	33	A (green), C (yellow)
reCj19314_126	Reverse	5’-GGCTCAGAGATTACCACCTCG-3’	133	(Anti-sense)	5′-7 T-TCTCTGTGTGCCGACGAAACCGAAGT-3’	33	A (green), C (yellow)
reCj19314_859	Forward	5’-GGTGGGCCTGTTTCCTATTCCG-3’	137	Reverse	5′-11 T-GGCCTCCATTGCAGACTTCTCAAACTG-3’	38	A (green), C (yellow)
reCj19314_859	Reverse	5’-GCAGTTTCATCATCCATTACGGC-3’	137	(Anti-sense)	5′-11 T-GGCCTCCATTGCAGACTTCTCAAACTG-3’	38	A (green), C (yellow)

ISSN: 1471-2164