Fig. 3

Analysis of translocated nad5 region in Anodonta cygnea. a Upper plot shows NAD5 protein alignment for members of family Unionoida (Anodonta anatina; KF030964), Mytiloida (Modiolus modiolus; KX821782) and Veneroida (Meretrix lusoria; ACV92129). Lower plot shows the same A. anatina sequence aligned to Homo sapiens and Bos taurus NAD5 sequences; UniProtKB code P03915 and PDB ID: 5LDW Chain L, respectively. In both upper and lower plots the region corresponding to the partial nad5 translocation in A. cygnea is annotated. Functional regions are coloured according to the legend and descriptions provided in InterPro for H. sapiens NAD5 (accession P03915). Amino acids in alignments are colour coded by similarity (black = 100% similar, dark gray = 80–100% similar, light grey = 60–80% similar, white = < 60% similar). Identity graphs were generated with a sliding-window size of 50 bp, furthermore red and purple colours along plots represent positions that have very low identities (30%), and less than complete identities (30% to < 100%), respectively. See methods for an explanation of how similarity was calculated. Images were generated in Geneious [22]. b Partial nad5 translocation in A. cygnea relative to the 3-dimentional structure of Respiratory Complex 1 (PDB ID: 5LDW). In particular, NAD5 (amino acid Chain L) is coloured. Note that structures are based on work in [26]