Fig. 1

Diagram representing the experimental design. a Seven life- stages were selected: embryos < 12 h after oviposition (Eggs 1, E1; corresponding to eggs of stage 1 following [1]), embryos within the last 12 h to be release as free swimming first instar juveniles (Eggs 3, E3; corresponding to embryos of stage 12 according to [1]); juvenile stage (J, animals of 4 days old), females F1, F2, F3 representing third brood females at the beginning, middle and end of their intermoult cycle, respectively. The final stage includes a reproductively active male (M) with 14 days. b images of the studied developmental stages, bars in each image are 1 mm. Further details about the description of life-stages are in methods section