Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content

Na, Wei; Wu, Yuan-Yuan; Gong, Peng-Fei; Wu, Chun-Yan; Cheng, Bo-Han; Wang, Yu-Xiang; Wang, Ning; Du, Zhi-Qiang; Li, Hui

doi:10.1186/s12864-018-4776-9

Table 2 Features of the 20 differentially expressed proteins identified by MALDI-TOF-MS

From: Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content

Stage	Protein spot No.	Fold change-ratio (Fat/Lean)	Accession No. (NCBInr/UniProt)	Protein name	Molecular weight (kDa)	pI	Protein score	Protein score C.I.%	Subcellular localization
E7	3	2.77	gi\|71895483	lysyl-tRNA synthetase	68,330.4	5.89	271	100	Cytoplasm; Mitochondrion
	4	2.35	Unknown protein
	9	1.54	Q9YHT1	Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial	74,024.6	6.65	63	94.987	Mitochondrion
	10	1.91	Q5ZJ08	Tyrosyl-tRNA synthetase, cytoplasmic	59,703.1	6.24	76	99.743	Cytoplasm
	12^a	1.65	gi\|326927880	PREDICTED: transketolase-like [Meleagris gallopavo]	87,487.4	8.17	125	100
	21^a	−1.67	gi\|326936041	PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial-like, partial [Meleagris gallopavo]	16,841.6	6.9	99	99.971
	23	1.99	gi\|57530789	Thioredoxin domain-containing protein 5	47,249.1	5.61	167	100
	27	−3.33	gi\|45384226	Sulfotransferase	36,333.5	5.89	129	100
	33	−1.97	gi\|45384222	Heat shock protein beta-1	21,715	5.77	135	100	Cytoplasm; Nucleus; Mitochondrion
	35^a	−1.62	gi\|109032822	PREDICTED: transcription factor BTF3-like [Macaca mulatta]	17,669.1	5.74	181	100
	40	4.02	O57535	Nucleoside diphosphate kinase	17,447.9	7.72	75	99.661	Cytoplasm; Cell membrane
	47	−1.56	gi\|50751047	PREDICTED: inosine triphosphate pyrophosphatase	22,533.4	5.8	142	100
	50	−1.53	gi\|50755667	PREDICTED: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10	20,769.2	5.98	181	100
	58	−1.6	O57535	Nucleoside diphosphate kinase	17,447.9	7.72	123	100	Cytoplasm; Cell membrane
E12	21^a	−2.13	gi\|326936041	PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial-like, partial [Meleagris gallopavo]	16,841.6	6.9	99	99.971
	24	−2.52	gi\|129293	Ovalbumin	43,195.6	5.19	197	100	Extracellular region
	25	−2.71	gi\|129293	Ovalbumin	43,195.6	5.19	370	100	Extracellular region
	27	−3.09	gi\|45384226	Sulfotransferase	36,333.5	5.89	129	100
	33	−1.67	gi\|45384222	Heat shock protein beta-1	21,715	5.77	135	100	Cytoplasm; Nucleus; Mitochondrion
E14	6	−1.89	Unknown protein
	7	−2.12	P84407	Alpha-fetoprotein	72,857.6	6.26	66	97.655	Secreted
	8	−2.39	P84407	Alpha-fetoprotein	72,857.6	6.26	71	99.187	Secreted
	14	−1.85	gi\|86129440	Coronin-1C	53,744.3	6,22	109	99.997	Intracellular
E17	31	−1.73	P08250	Apolipoprotein A-I	30,661.1	5.58	72	99.292	Secreted
E17	57	3.67	gi\|56119000	Intestinal fatty acid-binding protein	15,079.7	6.62	247	100	Intracellular part
E21	40	2.46	O57535	Nucleoside diphosphate kinase	17,447.9	7.72	75	99.661	Cytoplasm; Cell membrane

^arepresents proteins matched to other databases. Unknown protein indicates protein spots could not be identified

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ISSN: 1471-2164

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