Fig. 5

miR-14b modulate the polyembryony by targeting the RUNT, MBP-1 and KMT2E in M. cingulum. a-c Dual-luciferase reporter assays were performed to examine the interaction of mci-miR-14b and its target sequences and corresponding mutated targeting sequences (RUNT 3’UTR-Mt, MBP-1 CDS-Mt, KMT2E 3’UTR-Mt) cloned into the 3’UTR of the reporter gene. Signals from the reporters with the 3’UTR of RUNT, MBP-1, KMT2E were significantly knocked down in the presence of the mci-miR-14b mimics (p < 0.0001). Introducing the mutations at the target sites abolish the silence effects. ** indicates 0.001 < p < 0.05, *** indicates p < 0.001. d The target sites of mci-miR-14b appears in the 3’UTR of RUNT and KMT2E whereas in the CDS of MBP-1