Fig. 1From: Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fishCultivation, morphology and testicular histology of males determined via GSD and TSD. a Cultivation of males determined via GSD. b Cultivation of males determined via TSD. c-n Morphological masculine characteristics and testicular histology between males of GSD and TSD. c, i Body shape. d, j Gill cover. e, k Anus. f, l Testes. g, m Haematoxylin–eosin staining of testes. h, n Immunofluorescence staining of testes via CgVasa antibody. ♀: female; ♂: male; (+): with male-specific marker (MSM); (−): without MSMBack to article page