Fig. 4

Comparative analysis of the oviduct EVs protein content across the bovine estrous cycle. A) Principal Component analysis (PCA) on protein oviduct EVs content at 4 different stages of the estrus cycle (S1 in dark blue; S2 in light blue; S3 in brown and S4 in red) based on normalized weighted spectra data. B) Venn Diagram demonstrating relations among differential proteins (DP) for selected comparisons during the estrous cycle. Data were analyzed with cut-off FDR < 0.001. C) Dendrogram representing results of unsupervised hierarchical clustering (HCL) by MeV. Mean-centered expression values (log2 expression value of sample – mean of log2 expression values of all samples) for the samples of the 4 stages are shown. Rows indicate single DP, while columns represent different stages of the estrous cycle. Clusters of proteins strongly differentially regulated among stages are pointed with doted squares in HCL figure. These clusters of proteins were used for functional analysis and GO enriched terms are shown in proteins network in Fig. 5