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Table 2 The sensitivity and specificity of the heterozygous sites and Bayesian model-based clustering approaches for detecting mixed infection in artificial mixture and replicate samples. Calculations assume that the 4 technical replicates of one sample that were classified as mixed by the heterozygous sites method came from a pure sample. True positives were taken as the known artificially mixed Malawi samples (Table 1), and true negatives as the known pure Malawi samples (Table 1), and all H37Rv and Portuguese replicate strains (Additional file 1)

From: Identifying mixed Mycobacterium tuberculosis infections from whole genome sequence data

 

Number of mixed samples detected

Heterozygous sites method

Bayesian model-based clustering

Artificial mixed Malawi samples

33/36

27/36

Pure Malawi samples

0/12

0/12

Technical replicates

0/18

0/18

Biological replicates

4/32

0/32

Sensitivity of method

91.7%

75.0%

Specificity of method

93.5%

100%