Fig. 8
Enlargement of embryo cell during tartary buckwheat seed development. a, c, e, g and i Microscopic longitudinal sections of Tartary buckwheat seeds at 7, 13, 19, 25 and 30 DPA, respectively. b, d, f, h and j are the enlarged view of the boxes in (a), (c), (e), (g) and (i), respectively. SC (Seed cover). EM (Embryo). SL (Seminal leaf). The arrow represents the cell being measured. Bars = 100μm. (k) Embryo cell size at 7, 13, 19, 25 and 30 DPA. Error bars were obtained from five measurements. Small letter(s) above the bars indicate significant differences (α = 0.05, LSD) among treatments. (l) Subcellular location of DEGs related to embryo cell enlargement. This section provides information on the location in the cell (Graphics by Christian Stolte). m A simplified representation of the plant hormone signal transduction pathway of cell enlargement (adopted from the KEGG PATHWAY Database: http://www.genome.jp/kegg/pathway.html) shows the following: auxin influx carrier (AUX1), transport inhibitor response 1 (TIR1), auxin-responsive protein IAA (AUX/IAA), auxin response factor (ARF), auxin responsive GH3 gene family (CH3), and SAUR family protein (SAUR). The expression value of each gene is colored in log10(FPKM) in three samples X2 (13 DPA), X3 (19 DPA), and X4 (25 DPA)