Fig. 15

This figure provides the side-by-side comparison of gene expression levels determined by genome microarray analysis and their verification by qRT-PCR analyses for 15 candidate DEGs. This four panel figure provides visual contrasts of lipolytic (Panel a) and lipogenic (Panel b) genes and their differential expression determined by two independent analyses of 24 biological samples (12 embryos and 12 hatchlings). In Panel a, the normalized expression levels of five lipolytic DEGs from genome array analysis and time-course verification by qRT-PCR analysis are displayed in respective order, with genome array data in the top panel and its verification by qRT-PCR analysis in the bottom panel. Panel b shows hepatic expression patterns of seven lipogenic genes (THRSPA, SCD, ME1, FASN, ELOVL6, ACLY and HMGCL) as determined by genome array (top) and by qRT-PCR (bottom) analyses. Log scale expression levels were achieved by qRT-PCR analysis as shown in both bottom panels. Relative expression levels obtained by these two independent methods were used for Pearson’s correlation analysis and the determination of the Pearson’s correlation coefficient (see Additional file 8). Note: DIO1 was considered an invariant gene; therefore, DIO1 expression was not included in the Pearson’s Correlation Analysis